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Table of Contents

Table of Contents

Abstract
01. Abstract
Presentation
01. Introduction02. SPECT Image Processing03. PET Image Processing04. Patient Features at Baseline05. Voxelwise Exploratory SBR Analysis06. Regional DAT SBR Measures 07. Regional VMAT2 SBR Measures08. DAT & VMAT2 Sample Size Analysis09. Summary
Schematic of image processing PIANO DAT SPECT pipeline

The fully-automated PIANO™ SPECT pipeline for both exploratory voxelwise analysis and quantitative regional SBR analysis was used to investigate the Parkinson's disease populations.

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The DAT SPECT data was processed using our automated image processing "pipeline". PIANO™ is a configurable, modular, pipeline-based system for fully-automated processing of multi-modality images. PIANO™ is designed for high-throughput processing of large-scale, multi-center, neuroimaging data.

DAT SPECT scans first underwent:

- Reconstruction of the raw sinogram data using attenuation correction.

- Spatial filtering using a 3D Gaussian kernel with a full-width-at-half-maximum (FWHM) of 8 mm.

- Registration to the anatomical 3D T1-weighted MRI scan using a pseudo-DAT image generated from the anatomical scan.

Followed by:

- Regional analysis of the signal on pre-defined regions-of-interest (ROIs), warped from an atlas through the registration of the 3D T1-weighted scan to its corresponding anatomical template.

- SBR (specific binding ratio) was calculated as the ratio of each ROI to a reference region minus 1 to account for non-specific binding; the occipital cortex was chosen as the reference region because it has minimal DAT expression and is minimally affected by neurodegenerative diseases.

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